PSXV-8 Development of Imaging Techniques to Evaluate Dietary Interventions That Promote Regression of Osteochondrotic Lesions in a Growing Pig Model

Abstract The current objective was to develop imaging techniques characterize osteochondrotic-like lesions (OC-lesions) and evaluate the efficacy of dietary interventions promote lesion regression prevent lameness. Pigs (n=76) were weaned (~6kg) fed 1 2 nursery diets for 3-wk with no supplemental vitamin D, (-D, n=60) or 280 IU D3/kg (+D3, n=20). Following nursery, +D pigs standard (+Ctl) -D allotted 4 (15 pigs/TRT) until 24-wk: diet, D3; 25-OH-D3/kg, 25OH; 140 D3/kg+140 D3 + D3/kg+40ppm Mn, D3+Mn. euthanized at 8, 12, 24-weeks femurs dissected CT MRI scans. scans reconstructed in Mimics (Materialize software, Version 23) quantify whole-bone mineral volume (BMV; g/cm3) OC-lesion characteristics across growth plate articular surface (number, volume, area, location), as reported Table 1. images used validate detected CT. Differences gain feed intake not detected. At 8-wk, BMV reduced compared +Ctl; however, 12-wk increased 25OH D3+Mn pigs. By 24-wk all treatments different from +Ctl. No observed 8 wk. Number per bone +Ctl pigs, 3.6 vs. 1.7, respectively (P< 0.05). Summation tended increase D3+Mn, 81.2 97.0 mm3, respectively, 60.6 mm3. Limitations this study include low number observations incidence treatments, differences between Although inferences about nutrient are premature, we have developed methodologies better OC-lesions.